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shuweet! one of my poems is one of the semi-finalist in poetry.com. *shuweet*

~forget you not~

This is a very simple procedure on how to torture Escherichia Coli. Make sure you prepare everything right, or this will not work. and above all, have fun!

Ingredients:

1 TSA plate

bunsen burner

inoculating loop

1 test tube or TSA plate with E.coli culture

1 big  bottle of BLEACH

Aseptic technique is very important in this procedure. DO NOT FORGET THIS!! (Aseptic technique will be explained while i explain the steps)

Step 1. Turn on the bunsen burner. make sure you see the inner cone (blue flame). Hold the inoculating loop with your dominant hand. carefully burn the inoculating loop until it is searing red (this is to make sure that your inoculating loop is bacteria-free).

Step 2. Hold the test tube with the E.coli culture with your less dominant hand. open the test tube. heat the tip of the tube. now that the inoculating loop and the tube is free from all bacteria (except for e.coli), scrape a very small sample of the E.coli through your inoculating loop. Before you close the tube, reheat the tip of the tube then close it.

Step 3. Transfer the sample in your inoculating loop in to your  TSA plate. After this, make sure you burn the tip of the inoculating loop to ensure no E.coli will be spreading elsewhere.

Note: steps 1-3 is the aseptic technique

Step 4. Now that you have E.coli in your TSA plate, keep the plate in a 37 degree celsius temperature. this is to ensure optimal growth for the F*cking bastards!! i mean, the bacteria. Leave it for a week.

Step 5. retrieve the plate. Now the fun will begin.

Step 6. make sure your BIG bottle of bleach is ready. dip your inoculating loop in the bleach. now, make a zigzag pathway into the culture. (you just killed a million of them. Now that your inoculating loop is contaminated, dip your loop for a minute with bleach. (you just killed another million of them). now, scrape a very big sample of E.coli. Turn on your bunsen burner and BURN the HELL out of THEM. guess what?? you just killed a billion of them!! ~having fun yet?~

~You can keep on repeating step 6. however, if you get tired doing it over and over again, follow this final step.~

Final step. Open up your TSA plate. Open your big bottle of bleach. pour enough bleach to fill the whole plate (not too much so it would not overflow). now, wait for it… within 30 seconds or less, your TSA plate should be sparkling clean.

And there you have it. 6 easy steps on how to torture E.coli.

~Have Fun~

August 1, 2005 marks one of the best days of my life…

ahhh.. good times… good times…